Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

The study of the cell cycle focuses on mechanisms that regulate the timing and frequency of DNA duplication and cell division. As a biological concept, the cell cycle is defined as the period ...

Propidium iodide staining of DNA in fixed cells has been widely adopted in cell cycle analysis, but it stains all double-stranded nucleic acids, so cells must be treated with RNase before staining ...

When looking at cells with a microscope, the length of different stages of the cell cycle can be estimated using the formula: \(\text{Length of time in phase}=\) \(\frac{\text{observed number of ...

Calcineurin fine tunes the balance between proliferation and differentiation of Notch-responsive progenitors in the pancreatic ducts to enable beta cell regeneration in zebrafish.

The study addresses the complex regulation of HSCs, highlighting p21's multifunctional nature beyond cell cycle control. Through the use of p21-tdTomato mice, researchers differentiate between p21 ...

Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado Anschutz Medical Campus Wang, Hong (Co-PI) Division of Endocrinology, Metabolism and Diabetes, University of ...

A life cycle is the series of stages something passes through during its lifetime. We also use the term for things that are not living, like cell phones. The life cycle for anything you buy includes ...

This new kind of mice, that count with most of the human immune cell types with lower ...

This useful study by Gao et al identifies Hspa2 as a heterogeneous transcript in the early embryo and proposes a plausible mechanism showing interactions with Carm1. The authors propose that ...