Measurement of Cell Death in Mammalian Cells - PMC
2004年9月1日 · Assessment of nuclear morphology and membrane integrity using DAPI and PI is a valuable method for distinguishing between cell death mediated by apoptosis or necrosis. This approach can also be used for determining the stage of apoptotic cell death (early or late apoptosis).
Analysis of Apoptosis and Necroptosis by Fluorescence-Activated Cell ...
2016年4月1日 · DAPI is a fluorescent stain that binds strongly to A-T-rich regions in DNA. DAPI and PI only inefficiently pass through an intact cell membrane and, therefore, preferentially stain dead cells.
YAP-dependent necrosis occurs in early stages of Alzheimer’s …
2020年1月24日 · Here the authors show that YAP-mediated neuronal necrosis is increased in pre-symptomatic stages of Alzheimer’s disease and intervention to the necrosis rescues extracellular Aβ aggregation and...
Neutrophil-induced ferroptosis promotes tumor necrosis in ... - Nature
2020年10月27日 · Neutrophil depletion dampens necrosis. Neutrophils isolated from mouse brain tumors kill cocultured tumor cells. Mechanistically, neutrophils induce iron-dependent accumulation of...
Analysis of Apoptosis and Necroptosis by Fluorescence-Activated …
DAPI is a fluorescent stain that binds strongly to A-T-rich regions in DNA. DAPI and PI only inefficiently pass through an intact cell membrane and, therefore, preferentially stain dead cells.
Instead of PI, 4′,6-diamidino-2-phenylindole (DAPI) can also be used. DAPI is a uorescent stain that binds strongly fl to A-T-rich regions in DNA. DAPI and PI only inef ciently pass through an intact cell membrane and, fi therefore, preferentially stain dead cells.
Objective assessment of changes in nuclear morphology and cell ...
2014年5月12日 · Cells that were not incubated in staurosporine served as control. Caspase-3 expression in apoptotic cells was demonstrated by quantitative immunofluorescence. Nuclei were counterstained with DAPI. Assessments of nuclear morphology and cell distribution were performed using ImageJ software.
Apoptosis and necrosis: Detection, discrimination and phagocytosis ...
2008年3月1日 · A particular difficulty in defining necrosis is that in the absence of phagocytosis apoptotic cells become secondary necrotic cells with many morphological features of primary necrosis. In this review, we present a selection of techniques that can be used to identify necrosis and to discriminate it from apoptosis.
D-MAINS: A Deep-Learning Model for the Label-Free Detection of …
2024年6月8日 · Methods: Based on cellular morphology in phase contrast images, we developed a deep-learning model named Detector of Mitosis, Apoptosis, Interphase, Necrosis, and Senescence (D-MAINS). Results: D-MAINS utilizes machine learning and image processing techniques, enabling swift and label-free categorization of cell death, division, and senescence ...
Morphological assessment of apoptosis - ScienceDirect
2008年3月1日 · Caspase-dependent apoptosis mostly induces strong chromatin compaction in crescent shaped masses at the nuclear periphery, while caspase-independent apoptosis (sometimes called programmed necrosis) often results in lumpy, incomplete chromatin condensation (Fig. 3, reproduced with permission from [18] © (2001), The John Hopkins University Press ...