TUNEL assay - Wikipedia
Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3′- hydroxyl termini in the double-strand DNA breaks generated during apoptosis.
TUNEL staining methods and kits - Abcam
TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, also called the TUNEL assay, detects the DNA breaks formed when DNA fragmentation occurs in the last phase of apoptosis. Contents. Principles of TUNEL staining/TUNEL assay ; Different TUNEL staining/TUNEL assay methods
Detection of apoptosis by TUNEL assay - PubMed
Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis.
TUNEL Assays | Thermo Fisher Scientific - US
Explore our TUNEL assays for flow cytometry or imaging platforms. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assays are a widely used technique in the field of molecular biology and cell death research.
TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation
2021年1月2日 · Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3’-OH DNA termini) by endonucleases.
Click-iT TUNEL Alexa Fluor Imaging Assay Protocol
The Invitrogen Click-iT TUNEL Alexa Fluor imaging assays utilize a dUTP modified with an alkyne, a small, bio-orthogonal functional group that enables the nucleotide to be more readily incorporated by TdT than other modified nucleotides (Figures 1–2).
TUNEL Staining : The method of choice for measuring cell death
2022年3月4日 · We'll start by explaining what TUNEL staining is and how it works. Next, we'll discuss the different types of TUNEL stains available. Then, we'll take a look at the advantages of TUNEL staining over other apoptosis assays. Finally, we'll provide some tips for performing TUNEL staining correctly.
TUNEL Assay - an overview | ScienceDirect Topics
TUNEL Assay is a technique that allows for the detection of both single and double strand DNA breaks, using fluorescent or light microscopy, and can be coupled with flow cytometry for high-throughput analysis of thousands of samples, ensuring accuracy and reproducibility.
Identifying and Quantifying Apoptosis: Navigating Technical …
2003年4月1日 · Apoptosis is most commonly identified in tissue using terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL). TUNEL works on the principle that DNA strand breaks occur...
Detection of Apoptosis by TUNEL Assay | SpringerLink
2012年1月1日 · Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis.